Cloning and characterisation of chlorophyll synthase from Avena sativa

The chlorophyll synthase gene from oat (Avena sativa) was cloned and expressed in Escherichia coli. The deduced amino acid sequence consists of 378 amino acids including a presequence, of 46 amino acids. Deletion mutants show that a core protein comprising amino acid residues 88 to 377 is enzymatically active. The sequence of the mature protein shows 85% identity with the chlorophyll synthase of Arabidopsis thaliana and 62% identity with the chlorophyll synthase of Synechocystis PCC 6803. The gene is constitutively expressed as the same transcript level is found in dark-grown and in light-grown seedlings. The enzyme requires magnesium ions for activity; manganese ions can reconstitute only part of the activity. Diacetyl and N-phenylmaleimide (NPM) inhibit the enzyme activity. Site-directed mutagenesis reveals that, out of the 4 Arg residues present in the active core protein, Arg-91 and Arg-161 are essential for the activity. Five cysteine residues are present in the core protein, of which only Cys-109 is essential for the enzyme activity. Since the wild-type and all other Cys-mutants with the exception of the mutant C304A are inhibited by N-phenylmaleimide, we conclude that the inhibitor binds to a non-essential Cys residue to abolish activity. The role of the various Arg and Cys residues is discussed

Magnetoresistance, Micromagnetism and Domain Wall Effects in Epitaxial Fe and Co Structures with Stripe Domains

We review our recent magnetotransport and micromagnetic studies of lithographically defined epitaxial thin film structures of bcc Fe and hcp Co with stripe domains. Micromagnetic structure and resistivity anisotropy are shown to be the predominant sources of low field magnetoresistance (MR) in these microstructures, with domain wall (DW) effects smaller but observable (DW-MR $\lesssim 1$). In Fe, at low temperature, in a regime in which fields have a significant effect on electron trajectories, a novel negative DW contribution to the resistivity is observed. In hcp Co microstructures, temperature dependent transport measurements for current perpendicular and parallel to walls show that any additional resistivity due to DW scattering is very small.Comment: 7 pages, 8 figures, to appear in Journal of Applied Physics 199

Cosmological simulations of the circumgalactic medium with 1 kpc resolution: enhanced HI column densities

The circumgalactic medium (CGM), i.e. the gaseous haloes around galaxies, is both the reservoir of gas that fuels galaxy growth and the repository of gas expelled by galactic winds. Most cosmological, hydrodynamical simulations focus their computational effort on the galaxies themselves and treat the CGM more coarsely, which means small-scale structure cannot be resolved. We get around this issue by running zoom-in simulations of a Milky Way-mass galaxy with standard mass refinement and additional uniform spatial refinement within the virial radius. This results in a detailed view of its gaseous halo at unprecedented (1 kpc) uniform resolution with only a moderate increase in computational time. The improved spatial resolution does not impact the central galaxy or the average density of the CGM. However, it drastically changes the radial profile of the neutral hydrogen column density, which is enhanced at galactocentric radii larger than 40 kpc. The covering fraction of Lyman-Limit Systems within 150 kpc is almost doubled. We therefore conclude that some of the observational properties of the CGM are strongly resolution dependent. Increasing the resolution in the CGM, without increasing the resolution of the galaxies, is a promising and computationally efficient method to push the boundaries of state-of-the-art simulations.Comment: Accepted for publication in MNRAS Letters. Revised version: minor change

Desynchronization in diluted neural networks

The dynamical behaviour of a weakly diluted fully-inhibitory network of pulse-coupled spiking neurons is investigated. Upon increasing the coupling strength, a transition from regular to stochastic-like regime is observed. In the weak-coupling phase, a periodic dynamics is rapidly approached, with all neurons firing with the same rate and mutually phase-locked. The strong-coupling phase is characterized by an irregular pattern, even though the maximum Lyapunov exponent is negative. The paradox is solved by drawing an analogy with the phenomenon of ``stable chaos'', i.e. by observing that the stochastic-like behaviour is "limited" to a an exponentially long (with the system size) transient. Remarkably, the transient dynamics turns out to be stationary.Comment: 11 pages, 13 figures, submitted to Phys. Rev.

Vortices within vortices: hierarchical nature of vortex tubes in turbulence

The JHU turbulence database [1] can be used with a state of the art visualisation tool [2] to generate high quality fluid dynamics videos. In this work we investigate the classical idea that smaller structures in turbulent flows, while engaged in their own internal dynamics, are advected by the larger structures. They are not advected undistorted, however. We see instead that the small scale structures are sheared and twisted by the larger scales. This illuminates the basic mechanisms of the turbulent cascade.Comment: 2 pages, 1 low quality video, 1 high quality vide

Value pricing in the chemical industry:most powerful lever to profitability

The chemical industry in Europe is working hard on the improvement of their profitability base. But while innovation and complexity management are heavily discussed by the industry’s top managers, the most powerful lever to increase profitability is being ignored by many – value pricing. Arthur D. Little, jointly with Warwick Business School, conducted a pricing survey with the participation of managers from all chemical industry segments in which measures for profitability increases were investigated. Although a price increase of 1 % can lever the profit (EBIT) by 8 %, many companies focus on much weaker levers like reducing variable costs and sales volumes. In this article we look into the possible benefits of value pricing, the effective BASF approach and the problems posed by a customer management focussing on the perceived strategic importance of customers rather than their contribution margins

Direct observation of domain-wall configurations transformed by spin currents

Direct observations of current-induced domain-wall propagation by spin-polarized scanning electron microscopy are reported. Current pulses move head-to-head as well as tail-to-tail walls in sub-micrometer Fe_{20}Ni_{80} wires in the direction of the electron flow, and a decay of the wall velocity with the number of injected current pulses is observed. High-resolution images of the domain walls reveal that the wall spin structure is transformed from a vortex to a transverse configuration with subsequent pulse injections. The change in spin structure is directly correlated with the decay of the velocity.Comment: 5 pages, 3 figure

Asymptotic analysis of a secondary bifurcation of the one-dimensional Ginzburg-Landau equations of superconductivity

The bifurcation of asymmetric superconducting solutions from the normal solution is considered for the one-dimensional Ginzburg--Landau equations by the methods of formal asymptotics. The behavior of the bifurcating branch depends on the parameters d, the size of the superconducting slab, and $\kappa$, the Ginzburg--Landau parameter. The secondary bifurcation in which the asymmetric solution branches reconnect with the symmetric solution branch is studied for values of $(\kappa,d)$ for which it is close to the primary bifurcation from the normal state. These values of $(\kappa,d)$ form a curve in the $\kappa d$-plane, which is determined. At one point on this curve, called the quintuple point, the primary bifurcations switch from being subcritical to supercritical, requiring a separate analysis. The results answer some of the conjectures of [A. Aftalion and W. C. Troy, Phys. D, 132 (1999), pp. 214--232]